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Vol.46, No.3, 131 ~ 135, 2008
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| Title |
| Application of Nanoparticles for Materials Recognition Using Peptide Phage Display Technique -Part 2: Magnetic Bio-panning Using Fe3O4 Nanoparticles- |
| 이창우 Chang Woo Lee , 김민정 Min Jung Kim , 김세연 Se Yeon Kim , 좌용호 Yong Ho Choa , 이재성 Jai Sung Lee , R. Standaert , E. Owens , Jun Yan , M. Doktycz |
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| Abstract |
| The magnetism of Fe3O4 nanoparticles was applied to magnetic bio-panning process for finding specific sequences against Fe3O4 crystal phase. Vibrating sample magnetometer (VSM) measurement showed that the coercivity of 30 Oe and the saturation magnetization of 55 emu/g were sufficient in controlling particle movement and magnetizing particles in the media, respectively. This ferrimagnetism of nanoparticles practically enhanced panning efficiency by exaggerating centrifuge step and preventing particle loss. Sequencing results showed that histidine which was commonly found in peptide sequences played an important role in the binding onto Fe3O4 nanoparticle surface. However, various possible motifs were also observed from several neighboring amino acids of histidine. |
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| Key Words |
| Fe3O4 nanoparticles, magnetic bio-panning, phage display, histidine, materials recognition |
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